polyclonal rabbit anti-vimentin igg  (Synaptic Systems)

Journal: Annals of Translational Medicine

Article Title: GLI1 is increased in ovarian endometriosis and regulates migration, invasion and proliferation of human endometrial stromal cells in endometriosis

doi: 10.21037/atm.2019.10.76

Figure Lengend Snippet: Commercial sources and characteristics of antibodies used

Article Snippet: Characteristics of antibody used for immunohistochemistry are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Isotype/manufacture Product number Location IHC WB ICC GLI1 1:200 1:1,000 None Rabbit IgG (Polyclonal) ab49314/Abcam USA MMP2 None 1:750 None Rabbit IgG (Polyclonal) AF0577/Affinity UK MMP9 None 1:500 None Rabbit IgG (Polyclonal) AF5228/Affinity UK E-cadherin None None 1:50 Rabbit IgG (Polyclonal) ab40772/Abcam UK Vimentin None None 1:50 Rabbit IgG (Polyclonal) ab92547/Abcam UK GAPDH None 1:3,000 None Rabbit IgG (Polyclonal) 10494-1-AP/Proteintech USA Open in a separate window caption a8 Commercial sources and characteristics of antibodies used

Techniques:

Journal: Annals of Translational Medicine

Article Title: GLI1 is increased in ovarian endometriosis and regulates migration, invasion and proliferation of human endometrial stromal cells in endometriosis

doi: 10.21037/atm.2019.10.76

Figure Lengend Snippet: Commercial sources and characteristics of antibodies used

Article Snippet: Characteristics of antibody used for immunohistochemistry are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Isotype/manufacture Product number Location IHC WB ICC GLI1 1:200 1:1,000 None Rabbit IgG (Polyclonal) ab49314/Abcam USA MMP2 None 1:750 None Rabbit IgG (Polyclonal) AF0577/Affinity UK MMP9 None 1:500 None Rabbit IgG (Polyclonal) AF5228/Affinity UK E-cadherin None None 1:50 Rabbit IgG (Polyclonal) ab40772/Abcam UK Vimentin None None 1:50 Rabbit IgG (Polyclonal) ab92547/Abcam UK GAPDH None 1:3,000 None Rabbit IgG (Polyclonal) 10494-1-AP/Proteintech USA Open in a separate window caption a8 Commercial sources and characteristics of antibodies used Semi-quantitative analysis of immunohistochemical staining The analysis of immunohistochemistry results was independently carried out by two observers.

Techniques:

Journal: BioMed Research International

Article Title: Vimentin Overexpressions Induced by Cell Hypoxia Promote Vasculogenic Mimicry by Renal Cell Carcinoma Cells

doi: 10.1155/2019/7259691

Figure Lengend Snippet: Vimentin expressions in RCC cell lines. Vimentin protein (a) and mRNA (b) expressions were higher in 786-O and 769-P than those in OS-RC-2 under normoxia. Noteworthy, cell hypoxia induced by CoCl 2 treatment increased the expression of Vimentin on both mRNA and protein levels ( ∗∗ , P<0.01).

Article Snippet: After dewaxing, rehydration, and antigen retrieval, the slides were then incubated with polyclonal rabbit IgG anti-Vimentin antibody (1:100, CST, USA).

Techniques: Expressing

Journal: BioMed Research International

Article Title: Vimentin Overexpressions Induced by Cell Hypoxia Promote Vasculogenic Mimicry by Renal Cell Carcinoma Cells

doi: 10.1155/2019/7259691

Figure Lengend Snippet: The effects of siVimentin and cell hypoxia on 786-O and OS-RC-2. While downregulation of Vimentin reduced cell migration and invasion and VM structures, cell hypoxia can reverse these effects of siVimentin ((a), (c), and (e) showing OS-RC-2; (b), (d), and (f) showing 786-O; CH48, cell hypoxia for 48h; ns, not significant; ∗ , P<0.05; ∗∗ , P<0.01; ∗∗∗ , P<0.001).

Article Snippet: After dewaxing, rehydration, and antigen retrieval, the slides were then incubated with polyclonal rabbit IgG anti-Vimentin antibody (1:100, CST, USA).

Techniques: Migration

Journal: BioMed Research International

Article Title: Vimentin Overexpressions Induced by Cell Hypoxia Promote Vasculogenic Mimicry by Renal Cell Carcinoma Cells

doi: 10.1155/2019/7259691

Figure Lengend Snippet: The effects of cell hypoxia on molecular signals in 786-O and OS-RC-2. By western blot, cell hypoxia increased the protein expressions of Vimentin and AXL and reduced the protein expression of E-Cadherin; knockdown of Vimentin was accompanied with suppression of AXL and increase of E-Cadherin expressions (CH48, cell hypoxia for 48h; ns, not significant; ∗ , P<0.05; ∗∗ , P<0.01; ∗∗∗ , P<0.001).

Article Snippet: After dewaxing, rehydration, and antigen retrieval, the slides were then incubated with polyclonal rabbit IgG anti-Vimentin antibody (1:100, CST, USA).

Techniques: Western Blot, Expressing, Knockdown

Journal: BioMed Research International

Article Title: Vimentin Overexpressions Induced by Cell Hypoxia Promote Vasculogenic Mimicry by Renal Cell Carcinoma Cells

doi: 10.1155/2019/7259691

Figure Lengend Snippet: Relationship between VM and Vimentin expression and clinical parameters in ccRCC.

Article Snippet: After dewaxing, rehydration, and antigen retrieval, the slides were then incubated with polyclonal rabbit IgG anti-Vimentin antibody (1:100, CST, USA).

Techniques: Expressing, Staining

Journal: BioMed Research International

Article Title: Vimentin Overexpressions Induced by Cell Hypoxia Promote Vasculogenic Mimicry by Renal Cell Carcinoma Cells

doi: 10.1155/2019/7259691

Figure Lengend Snippet: (a) VM phenomena in ccRCC tissues. The red arrows indicated microvessels with CD34 positive staining and the blue arrows indicated VM structures with CD34 negative and PAS positive staining. (b) Vimentin expressions in ccRCC tissues. Showing high expression of Vimentin (left) and low expression of Vimentin in ccRCC. (c) Both VM (left) and Vimentin expressions (right) were correlated with survival of ccRCC patients.

Article Snippet: After dewaxing, rehydration, and antigen retrieval, the slides were then incubated with polyclonal rabbit IgG anti-Vimentin antibody (1:100, CST, USA).

Techniques: Staining, Expressing